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total stat1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc total stat1
    p38 enhances Src-mediated TAB1-Y481 phosphorylation. A and C , COS-7 cells were transfected with EGFP-TAB1, Src, and the wild-type (WT) or kinase-dead (KD) mutant of Flag-p38α. SB203580 (10 μM) was added for 5 h ( C ). B , The relative quantification of pY481-TAB1, normalized to total TAB1, is presented as the mean ± SD of three independent experiments. D – F , COS-7 cells transfected with EGFP-TAB1 (WT or YF) were stimulated with 1 mM H 2 O 2 for the indicated time. SB203580 was added as a pre-treatment for 5 h. G , COS-7 cells were pretreated with baricitinib (0.5 μM), SB203580 (10 μM), and saracatinib (0.5 μM) for 1 h and then treated with 1 mM H 2 O 2 for 5 min. H , The relative quantification of <t>pY701-STAT1,</t> normalized <t>to</t> <t>total-STAT1,</t> is presented as the mean ± SD of three independent experiments. Cell lysates were immunoblotted with primary antibodies indicated ( A and C – G ). p values were calculated by one-way ANOVA followed by either Tukey’s HSD test was applied. ∗ p < 0.05, ∗∗ p < 0.01.
    Total Stat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+stat1/pmc12925544-188-13-23
    Average 86 stars, based on 1 article reviews
    total stat1 - by Bioz Stars, 2026-09
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    1) Product Images from "The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1"

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2026.111200

    p38 enhances Src-mediated TAB1-Y481 phosphorylation. A and C , COS-7 cells were transfected with EGFP-TAB1, Src, and the wild-type (WT) or kinase-dead (KD) mutant of Flag-p38α. SB203580 (10 μM) was added for 5 h ( C ). B , The relative quantification of pY481-TAB1, normalized to total TAB1, is presented as the mean ± SD of three independent experiments. D – F , COS-7 cells transfected with EGFP-TAB1 (WT or YF) were stimulated with 1 mM H 2 O 2 for the indicated time. SB203580 was added as a pre-treatment for 5 h. G , COS-7 cells were pretreated with baricitinib (0.5 μM), SB203580 (10 μM), and saracatinib (0.5 μM) for 1 h and then treated with 1 mM H 2 O 2 for 5 min. H , The relative quantification of pY701-STAT1, normalized to total-STAT1, is presented as the mean ± SD of three independent experiments. Cell lysates were immunoblotted with primary antibodies indicated ( A and C – G ). p values were calculated by one-way ANOVA followed by either Tukey’s HSD test was applied. ∗ p < 0.05, ∗∗ p < 0.01.
    Figure Legend Snippet: p38 enhances Src-mediated TAB1-Y481 phosphorylation. A and C , COS-7 cells were transfected with EGFP-TAB1, Src, and the wild-type (WT) or kinase-dead (KD) mutant of Flag-p38α. SB203580 (10 μM) was added for 5 h ( C ). B , The relative quantification of pY481-TAB1, normalized to total TAB1, is presented as the mean ± SD of three independent experiments. D – F , COS-7 cells transfected with EGFP-TAB1 (WT or YF) were stimulated with 1 mM H 2 O 2 for the indicated time. SB203580 was added as a pre-treatment for 5 h. G , COS-7 cells were pretreated with baricitinib (0.5 μM), SB203580 (10 μM), and saracatinib (0.5 μM) for 1 h and then treated with 1 mM H 2 O 2 for 5 min. H , The relative quantification of pY701-STAT1, normalized to total-STAT1, is presented as the mean ± SD of three independent experiments. Cell lysates were immunoblotted with primary antibodies indicated ( A and C – G ). p values were calculated by one-way ANOVA followed by either Tukey’s HSD test was applied. ∗ p < 0.05, ∗∗ p < 0.01.

    Techniques Used: Phospho-proteomics, Transfection, Mutagenesis, Quantitative Proteomics



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    p38 enhances Src-mediated TAB1-Y481 phosphorylation. A and C , COS-7 cells were transfected with EGFP-TAB1, Src, and the wild-type (WT) or kinase-dead (KD) mutant of Flag-p38α. SB203580 (10 μM) was added for 5 h ( C ). B , The relative quantification of pY481-TAB1, normalized to total TAB1, is presented as the mean ± SD of three independent experiments. D – F , COS-7 cells transfected with EGFP-TAB1 (WT or YF) were stimulated with 1 mM H 2 O 2 for the indicated time. SB203580 was added as a pre-treatment for 5 h. G , COS-7 cells were pretreated with baricitinib (0.5 μM), SB203580 (10 μM), and saracatinib (0.5 μM) for 1 h and then treated with 1 mM H 2 O 2 for 5 min. H , The relative quantification of pY701-STAT1, normalized to total-STAT1, is presented as the mean ± SD of three independent experiments. Cell lysates were immunoblotted with primary antibodies indicated ( A and C – G ). p values were calculated by one-way ANOVA followed by either Tukey’s HSD test was applied. ∗ p < 0.05, ∗∗ p < 0.01.

    Journal: The Journal of Biological Chemistry

    Article Title: The stress-activated kinase p38 mediates non-canonical activation of Src and tyrosine phosphorylation of the adapter protein TAB1

    doi: 10.1016/j.jbc.2026.111200

    Figure Lengend Snippet: p38 enhances Src-mediated TAB1-Y481 phosphorylation. A and C , COS-7 cells were transfected with EGFP-TAB1, Src, and the wild-type (WT) or kinase-dead (KD) mutant of Flag-p38α. SB203580 (10 μM) was added for 5 h ( C ). B , The relative quantification of pY481-TAB1, normalized to total TAB1, is presented as the mean ± SD of three independent experiments. D – F , COS-7 cells transfected with EGFP-TAB1 (WT or YF) were stimulated with 1 mM H 2 O 2 for the indicated time. SB203580 was added as a pre-treatment for 5 h. G , COS-7 cells were pretreated with baricitinib (0.5 μM), SB203580 (10 μM), and saracatinib (0.5 μM) for 1 h and then treated with 1 mM H 2 O 2 for 5 min. H , The relative quantification of pY701-STAT1, normalized to total-STAT1, is presented as the mean ± SD of three independent experiments. Cell lysates were immunoblotted with primary antibodies indicated ( A and C – G ). p values were calculated by one-way ANOVA followed by either Tukey’s HSD test was applied. ∗ p < 0.05, ∗∗ p < 0.01.

    Article Snippet: Primary antibodies for pY419-Src family kinase (2101S), total-Src (2110S), pT180/pY182-p38 (4511S), pY701-STAT1 (sc-136229), total-STAT1 (sc-346), pY576/577-FAK (3281S), and total-FAK (3285S) were purchased from Cell Signaling Technology. pS75-Src (ab79308) was from Abcam.

    Techniques: Phospho-proteomics, Transfection, Mutagenesis, Quantitative Proteomics